Determination of betaxolol in plasma by high-performance liquid chromatography with fluorescence detection.

Objective. To present a liquid chromatography with fluorescence detection method for the determination of betaxolol in human plasma without internal standard. Method. A reversed-phase column was used with a fluorescence detector at 227 nm (excitation) and 301 nm (emission). The mobile phase was consisted of acetonitrile, methanol and phosphate buffer at a flow rate of 1.0 ml/min. Results. The standard curve was linear over a range of 2.5 – 80 ng/ml. The recovery from human plasma was found to be 88% of betaxolol. Validation results showed the linearity, specificity, accuracy, precision and stability, as well as on application to the analysis of plasma samples 1 hour after oral administration of 40 mg of betaxolol in healthy volunteers. Conclusion. Liquid chromatography with fluorescence detection is an accurate, precise and reliable method to determine the betaxolol hydrochloride in human plasma. [Life Science Journal. 2007; 4(3): 30 – 33] (ISSN: 1097 – 8135).